Related Experiment Video
Updated: Aug 15, 2026

08:34
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
Published on: February 5, 2020
Immobilization of enzymes on a microchannel surface through cross-linking polymerization
Takeshi Honda1, Masaya Miyazaki, Hiroyuki Nakamura
1Nanotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 807-1 Shuku, Tosu, Saga 841-0052, Japan.
Abstract:
A novel and facile method for the preparation of an enzyme-immobilized microreactor has been developed in which enzymes are immobilized as an enzyme-polymer membrane formed on the inner wall of the microchannel by a cross-linking polymerization method; the resulting microreactor shows excellent reaction performance and stability against denaturating agents.

