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Updated: Aug 15, 2026

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
Published on: April 8, 2016
[Androgen and estrogen receptors in intact, hyperplastic, peritumorous and malignant prostate tissues: a link to
Abstract:
A total of 134 samples of intact prostate tissue, nodular hyperplasia and prostate cancer were examined. Number of androgen-positive cells was 28.51 +/- 3.50%, Ki-67 expression index - 2.76 +/- 0,45% and Bcl-2--6.25 +/- 1.38% in nodular hyperplastic tissue. In peritumorous epithelial tissue, that parameter dropped to 20.60 +/- 3, 10%, while stromal E+ cells index rose to 19.42 +/- 4, 81. Androgen expression varied 40-90% in tumor epithelium and that of Ki-67--8.26-13.93. Bcl-2 expression was lower in recurrent tumor (4.95 +/- 2.76) as compared with adenocarcinoma microfoci (5.41 +/- 0.71).
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