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Updated: Aug 15, 2026

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
Published on: August 22, 2019
Spectral unmixing of flavin autofluorescence components in cardiac myocytes
D Chorvat1, J Kirchnerova, M Cagalinec
1Department of Biophotonics, International Laser Centre, Ilkovicova 3, 81219 Bratislava, Slovakia. dusan@ilc.sk
Abstract:
We applied linear unmixing approach to reveal individual components of intrinsic flavin fluorescence signal recorded in living cardiac cells by spectrally resolved confocal microscopy. Responses of whole-cell autofluorescence to modulators of cell metabolism and respiration were used as a tool of separation of its components; their spectral profiles, estimated by principal component analysis, correspond to free FAD and FAD bound to different enzymes of electron transport chain.

