Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunoreactive dynorphin in rat tissues and plasma.

S Spampinato1, A Goldstein

  • 1Research Foundation, Palo Alto, CA 94304, USA.

Neuropeptides
|January 1, 1983
PubMed
Summary

Immunoreactive dynorphin (ir-dyn) is present in various rat tissues, including the gastrointestinal tract, heart, and skeletal muscle. This peptide exhibits a larger molecular weight than expected, suggesting novel forms of dynorphin exist in peripheral tissues.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficacy of self-management program associated with a spa therapy for knee osteoarthritis patients (GETT 2): a research protocol for a randomized trial.

Trials·2023
Same author

A bright γ-ray flare interpreted as a giant magnetar flare in NGC 253.

Nature·2021
Same author

Involvement of the Spinal Cord in Primary Mitochondrial Disorders: A Neuroimaging Mimicker of Inflammation and Ischemia in Children.

AJNR. American journal of neuroradiology·2021
Same author

Use of Co<sup>2+</sup>:MgAl<sub>2</sub>O<sub>4</sub> transparent ceramics in passive Q-switching of an Er:Glass laser at 1.534 µm.

Optics express·2020
Same author

The Perirolandic Sign: A Unique Imaging Finding Observed in Association with Polymerase γ-Related Disorders.

AJNR. American journal of neuroradiology·2020
Same author

Prediction-based neural mechanisms for shielding the self from existential threat.

NeuroImage·2019

Area of Science:

  • Neuroscience
  • Endocrinology
  • Gastroenterology

Background:

  • Dynorphin (dyn) is a key opioid peptide found in the central nervous system.
  • Previous studies have reported dyn distribution in the pituitary, brain, and spinal cord.
  • The presence and characteristics of dyn in peripheral tissues remain less understood.

Purpose of the Study:

  • To determine the distribution of immunoreactive dynorphin (ir-dyn) in various rat tissues.
  • To characterize the molecular form of ir-dyn in peripheral tissues and plasma.
  • To investigate the influence of vagotomy, denervation, hypophysectomy, and adrenalectomy on ir-dyn levels.

Main Methods:

  • Radioimmunoassay using a specific antiserum for ir-dyn quantification.
  • Gel permeation chromatography for molecular weight analysis.
  • Octadecylsilyl-silica cartridge extraction for plasma ir-dyn concentration.

Main Results:

  • Highest ir-dyn concentrations were observed in the pituitary, brain, and spinal cord.
  • Significant ir-dyn levels were also detected in the stomach, upper intestine, heart, and skeletal muscle.
  • ir-dyn in gastrointestinal tract, heart, and skeletal muscle showed a higher apparent molecular weight than standard dynorphin.
  • Plasma ir-dyn appeared in two peaks with higher molecular weights, unaffected by hypophysectomy or adrenalectomy.
  • Unilateral vagotomy and denervation did not alter ir-dyn levels in tested tissues.

Conclusions:

  • Immunoreactive dynorphin is widely distributed in rat peripheral tissues, not just the central nervous system.
  • Peripheral ir-dyn exists in forms with higher molecular weights than the canonical dynorphin.
  • These novel dynorphin forms in peripheral tissues are independent of vagal/splanchnic innervation and major endocrine glands.

Related Experiment Videos