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Surface plasmon resonance phase-shift interferometry: real-time DNA microarray hybridization analysis.

Shean-Jen Chen1, Yuan-Deng Su, Feng-Ming Hsiu

  • 1National Cheng Kung University, Department of Engineering Science, Tainan 701, Taiwan. sheanjen@mail.ncku.edu.tw

Journal of Biomedical Optics
|October 19, 2005
PubMed
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This study introduces a novel Surface Plasmon Resonance (SPR) phase-shift interferometry (PSI) imaging system for label-free DNA hybridization detection. The system achieves high resolution and quantitative analysis of biomolecular interactions on microarrays.

Area of Science:

  • Biophotonics
  • Biosensing
  • Surface Plasmon Resonance Imaging

Background:

  • Biomolecular interaction analysis is crucial for diagnostics and drug discovery.
  • Label-free detection methods reduce experimental complexity and cost.
  • Surface Plasmon Resonance (SPR) is a sensitive technique for monitoring binding events.

Purpose of the Study:

  • To develop and demonstrate a novel Surface Plasmon Resonance (SPR) phase-shift interferometry (PSI) imaging system.
  • To achieve high-resolution, label-free detection of DNA hybridization.
  • To provide quantitative analysis of biomolecular interactions on microarrays.

Main Methods:

  • Combined Surface Plasmon Resonance (SPR) with modified Mach-Zehnder phase-shift interferometry (PSI).
  • Developed an SPR-PSI imaging system for spatial phase variation measurement.

Related Experiment Videos

  • Utilized microarray chips for DNA hybridization experiments.

    • Main Results:

    • Achieved an enhanced detection limit of 2.5 x 10(-7) refractive index change.
    • Demonstrated long-term phase stability of pi/100 in 30 min.
    • Obtained a spatial phase resolution of pi/300 over a 100 x 100 microm2 area.
    • Successfully performed label-free observation of 15-mer DNA microarray hybridization.

    Conclusions:

    • SPR-PSI imaging is a powerful, label-free technique for high-throughput screening of biomolecular interactions.
    • The system offers high resolution and quantitative data for DNA hybridization analysis.
    • This technology has potential applications in diagnostics and molecular screening.