Biocompatible zeolite-dye composites with anti-amyloidogenic properties.

Area of Science:

• Biomedicine and Pharmacy
• Materials Science
• Nanotechnology

Background:

• Multifunctional materials are attractive in biomedicine and pharmacy.
• Mesoporous clinoptilolite (CZ) can serve as a carrier for targeted drug delivery.
• Dye-clinoptilolite composites are fabricated using aluminum phthalocyanine, zinc porphine, and hypericin.

Purpose of the Study:

• To investigate the anti-amyloidogenic effects of pure dyes and CZ-dye composites on insulin amyloidogenesis.
• To compare the efficacy of different dyes and their incorporation into CZ.
• To evaluate the biocompatibility and biodistribution of CZ-dye composites.

Main Methods:

• Fabrication of clinoptilolite-dye composites.
• Assessment of effects on insulin amyloid fibril formation and disassembly.
• In vitro studies using U87 MG cells.
• In vivo studies using the quail chorioallantoic membrane model.

Main Results:

• All tested dyes and CZ-dye composites affected insulin amyloidogenesis.
• Aluminum phthalocyanine exhibited the strongest anti-amyloidogenic activity, suggesting structure-dependent effects.
• Incorporation into CZ enhanced the anti-amyloidogenic activity of the dyes.
• CZ-dye composites demonstrated good cell metabolic activity, biocompatibility, and fluorescence biodistribution.

Conclusions:

• Clinoptilolite-dye composites possess significant anti-amyloidogenic potential.
• Aluminum phthalocyanine shows promising structurally-dependent anti-amyloidogenic activity.
• These composites are suitable for drug delivery, photodiagnostics, and photodynamic therapy, with potential for enhanced therapeutic effects via photodynamic action.