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Updated: Aug 15, 2026

Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
12:38

Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction

Published on: February 15, 2018

Determination of picomolar equilibrium dissociation constants in solution by enzyme-linked immunosorbent assay with

Katrina High1, Yuan Meng, Michael W Washabaugh

  • 1Department of Bioprocess and Bioanalytical Research, Merck Research Laboratories, West Point, PA 19486, USA.

Analytical Biochemistry
|October 19, 2005
PubMed
Abstract

No abstract available in PubMed .

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Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
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The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium: