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Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
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Synthesis and application of mono-6-ammonium-6-deoxy-beta-cyclodextrin chloride as chiral selector for capillary

Weihua Tang1, I Wayan Muderawan, Siu Choon Ng

  • 1Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.

Journal of Chromatography. A
|October 19, 2005
PubMed

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Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
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Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...
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