9-Hydroxybenfluron: effect on energy-yielding processes in Ehrlich ascites and P388 murine leukemia cells

The main purpose of the present investigation was to study the effect of 9-hydroxybenfluron (HBF) on aerobic glucose consumption, lactic acid formation, content of total (T-SH) and non-protein thiol groups (NP-SH), endogenous respiration and levels of ATP in both Ehrlich ascites and P388 murine leukemia cells. The lowest concentrations of HBF significantly stimulated both glucose consumption and lactate formation in Ehrlich ascites cells. HBF decreased the level of both T-SH and NP-SH in Ehrlich cells. However, the decrease in the level of NP-SH was significantly higher. Both respiration and ATP levels were inhibited more markedly in Ehrlich than in P388 cells. In P388 cells a significant decrease in ATP level (67%) was noted only at the highest concentration of HBF (100 mumol/l).