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Convergent solid-phase peptide synthesis IX: application to the synthesis of peptides with repetitive sequences
C Celma1, F Albericio, E Pedroso
1Departament de Química Orgànica, Universitat de Barcelona, Spain.
Peptide Research
|January 1, 1992
Abstract:
The synthesis of a 36 amino acid peptide containing six conserved repeats of Val-His-Leu-Pro-Pro-Pro which corresponds to the glutelin-2 protein of maize has been carried out using a convergent approach. The protected single sequence repeat has been synthesized using a combination of a 9-fluorenylmethyloxycarbonyl (Fmoc) protection scheme and a p-alkoxybenzyl resin. The final peptide, as well as the different intermediate peptides, has been characterized by fast atom bombardment mass spectrometry (FAB-MS) and by co-elution with another sample obtained by stepwise continuous flow synthesis.