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Updated: Aug 15, 2026

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Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
In vitro construction and screening of a Burkholderia cepacia lipase library using single-molecule PCR and cell-free
Yuichi Koga1, Kouei Kobayashi, Junhao Yang
1Laboratory of Molecular Biotechnology, Graduate School of Biological & Agricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Journal of Bioscience and Bioengineering
|October 20, 2005
Abstract:
A mutant library of Burkholderia cepacia lipase KWI-56 was constructed on microplates by a cell-free process and tested with a chromogenic assay. This high-throughput construction system can be used to screen mutant proteins based on their catalytic activity.

