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Analysis of estrogen-like compounds in the environment by high performance liquid chromatography bioassay

Seiji Iwasaki1, Susumu Kato, Masaaki Takahashi

  • 1Mie Pref. Institute of Public Health & Environmental Science, 3690-1 Sakura, Yokkaichi, Mie 512-1211, Japan. iwasas00@pref.mie.jp

Journal of Bioscience and Bioengineering
|October 20, 2005
PubMed

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...