Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

On-chip magnetic bead microarray using hydrodynamic focusing in a passive magnetic separator.

K Smistrup1, B G Kjeldsen, J L Reimers

  • 1MIC-Department of Micro and Nanotechnology, Technical University of Denmark, Building 345east, DK-2800 Kongens Lyngby, Denmark. krs@mic.dtu.dk

Lab on a Chip
|October 20, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ecophysiology and niche differentiation of three genera of polyphosphate-accumulating bacteria in a full-scale wastewater treatment plant.

mSystems·2025
Same author

Gendered analysis of care work burden and mental health using data from the Gutenberg Covid-19 study.

Scientific reports·2025
Same author

Wnt1's Differential Effects on Craniofacial Bone and Tooth Development.

Journal of dental research·2025
Same author

Arterial stiffness assessment in coronary microvascular dysfunction and heart failure with preserved ejection fraction: An initial report from the WISE-CVD continuation study.

American heart journal plus : cardiology research and practice·2024
Same author

Coronary atheroma burden predicts flow reserve in women with ischemia and nonobstructive coronary artery disease.

American heart journal plus : cardiology research and practice·2024
Same author

Linking the Mechanics of Chewing to Biology of the Junctional Epithelium.

Journal of dental research·2023

This study presents a novel method for functionalizing microfluidic chips using magnetic beads and hydrodynamic focusing. This technique enables precise, repeatable, and crosstalk-free immobilization of probes for sensitive DNA detection.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Biomolecular Engineering

Background:

  • Lab-on-a-chip systems face challenges with heat and chemical sensitivity for DNA and protein microarrays.
  • Developing robust and versatile microfluidic functionalization methods is crucial for advanced diagnostics.

Purpose of the Study:

  • To develop a novel method for functionalizing microchannels with two types of magnetic beads for multiplexed biomolecular assays.
  • To demonstrate the selective capture and repeated functionalization of microfluidic devices without cross-contamination.

Main Methods:

  • Utilized hydrodynamic focusing and a passive magnetic separator with soft magnetic elements for bead manipulation.
  • Introduced two types of functionalized magnetic beads and a barrier flow simultaneously into a microchannel.

Related Experiment Videos

  • Employed fluorescence-labeled beads and on-chip hybridization experiments to validate the method.

    • Main Results:

    • Successfully captured two distinct types of magnetic beads at designated channel sidewalls.
    • Demonstrated selective DNA hybridization, including recognition of single base pair mismatches.
    • Showcased repeatable microchannel functionalization and cleaning with no observed cross-talk between experiments.

    Conclusions:

    • The developed method offers a heat- and chemical-resistant approach for microfluidic chip functionalization.
    • This technique enables sensitive and multiplexed detection of nucleic acid targets.
    • The system's reusability and lack of cross-talk make it suitable for diverse lab-on-a-chip applications.