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Related Concept Videos

Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

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Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
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Canonical Wnt Signaling Pathway02:54

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The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
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Pleiotropy01:33

Pleiotropy

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Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
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Determination01:51

Determination

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During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
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Teeth01:15

Teeth

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The formation of teeth, also known as odontogenesis, is a complex process that begins in utero, around the sixth week of embryonic development. There are three stages to this process: the bud stage, the cap stage, and the bell stage.
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Notch Signaling Pathway03:14

Notch Signaling Pathway

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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
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Related Experiment Video

Updated: Jan 18, 2026

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy
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Wnt1's Differential Effects on Craniofacial Bone and Tooth Development.

R Mahmoud1, A Simon1, J Luther2

  • 1Department of Orthodontics, University of Leipzig Medical Center, Leipzig, Germany.

Journal of Dental Research
|June 2, 2025
PubMed
Summary
This summary is machine-generated.

Wnt1 signaling impacts craniofacial bone and tooth development differently based on timing and origin. Early Wnt1 activation affects bone, while prolonged activation causes severe defects and suppresses osteoclast activity.

Keywords:
Wnt1 activationenamelosteoanabolicosteoclastogenesisosteopetrosis-like pathologytransgenic mouse model

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Analysis of Craniomaxillofacial Malformations in Mice Using Three-dimensional Microcomputed Tomography
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Analysis of Craniomaxillofacial Malformations in Mice Using Three-dimensional Microcomputed Tomography
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Area of Science:

  • Developmental Biology
  • Genetics
  • Craniofacial Biology

Background:

  • The Wnt signaling pathway is crucial for craniofacial bone and tooth development.
  • Specific Wnt variants and their precise roles, including Wnt1, are still being investigated.

Purpose of the Study:

  • To investigate the role of Wnt1 in craniofacial bone and tooth development using a mouse model.
  • To analyze the effects of Wnt1 activation at different developmental stages.

Main Methods:

  • Utilized single-cell sequencing data from mouse incisors.
  • Employed a Col1a1-dependent Wnt1 transgenic mouse model for Wnt1 induction.
  • Performed 3D micro-computed tomography and bulk RNA sequencing, alongside in vitro experiments.

Main Results:

  • Early Wnt1 induction affected craniofacial bone but not initial tooth development.
  • Prolonged embryonic Wnt1 induction led to mortality, osteopetrosis-like bone overgrowth, and tooth malformations.
  • Postnatal Wnt1 activation impaired tooth root formation, odontoblast differentiation, and suppressed osteoclastogenesis dose-dependently.
  • Wnt1 activation increased neural crest-derived craniofacial bone volume but not mesenchymal-derived bone.

Conclusions:

  • Wnt1 exhibits differential effects on craniofacial bone development depending on its cellular origin.
  • Wnt1 plays a significant role in modulating osteoclast activity.
  • Findings suggest Wnt1's broader implications for craniofacial development and potential therapeutic strategies.