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Updated: Aug 15, 2026

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
Published on: April 29, 2022
Determination of protein phosphorylation sites by mass spectrometry: a novel electrospray-based method
Vera Alverdi1, Francesca Di Pancrazio, Giovanna Lippe
1Department of Biomedical Sciences and Technologies, Center of Excellence M.A.T.I., University of Udine, P. le Kolbe 4, 33100 Udine, Italy.
Abstract:
Several methods are used to identify protein phosphorylation sites. We report a novel electrospray-based method for the determination of phosphorylation sites by mass spectrometry, using two different declustering potential values. This method allows one to obtain, with a single liquid chromatography/mass spectrometry (LC/MS) run, the pattern with either the phosphorylated or the unphosphorylated species of a protein tryptic digest, that can be further analyzed by tracing back the origin of each HPO3-deprived form using the capabilities of tandem mass spectrometers.
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