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Related Experiment Videos

Pyelonephritic Escherichia coli expressing P fimbriae decrease immune response of the mouse kidney.

James C Rice1, Tao Peng, Jeff S Spence

  • 1Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX 77555-0562, USA. jrice@utmb.edu

Journal of the American Society of Nephrology : JASN
|October 21, 2005
PubMed
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P fimbriae on E. coli reduce kidney polymeric Ig receptor (pIgR) and urinary IgA, aiding bacterial persistence in urinary tract infections. This mechanism helps uropathogenic E. coli evade immune defenses.

Area of Science:

  • Microbiology
  • Immunology
  • Urology

Background:

  • P fimbriae are key virulence factors of uropathogenic Escherichia coli (E. coli), mediating adherence to the urinary tract.
  • Ascending urinary tract infections (UTIs) are common, particularly in women, with P fimbriated E. coli being a major cause.
  • The polymeric Ig receptor (pIgR) on renal epithelia transports IgA into the urine, a crucial part of mucosal immunity.

Purpose of the Study:

  • To investigate if P fimbriae on uropathogenic E. coli regulate renal epithelial pIgR expression and IgA transport.
  • To determine the role of P fimbriae in establishing ascending pyelonephritis by modulating the host immune response.

Main Methods:

  • A mouse model of ascending pyelonephritis was used, infecting with P fimbriated (P+) and non-fimbriated (P-) E. coli.

Related Experiment Videos

  • Kidney pIgR mRNA and protein levels were quantified.
  • Urinary IgA levels were measured.
  • Experiments were also conducted in LPS-hyporesponsive mice.

    • Main Results:

    • P(+) E. coli established infection and persisted at higher levels than P(-) E. coli.
    • Infection with P(+) E. coli led to a significant downregulation of kidney pIgR mRNA and protein starting at 48 hours post-infection.
    • This decrease in pIgR correlated with reduced urinary IgA levels in P(+)-infected mice.
    • pIgR downregulation was also observed in LPS-hyporesponsive mice infected with P(+) E. coli.

    Conclusions:

    • P fimbriae represent a novel virulence mechanism for E. coli.
    • P fimbriae downregulate renal pIgR expression, leading to decreased IgA transport into the urinary space.
    • This immune evasion strategy likely contributes to the establishment and persistence of ascending pyelonephritis caused by P fimbriated E. coli.