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Related Experiment Videos

sigmaB activity in a Staphylococcus aureus hemB mutant.

Maria M Senn1, Markus Bischoff, Christof von Eiff

  • 1Department of Medical Microbiology, University of Zürich, Gloriastrasse 32, 8006 Zürich, Switzerland.

Journal of Bacteriology
|October 21, 2005
PubMed
Summary

Inactivating hemB in Staphylococcus aureus causes a small-colony variant with altered global regulator expression. This study reveals permanent sigmaB activity and modified virulence factor control in the hemB mutant.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacterial Genetics

Background:

  • Staphylococcus aureus is an opportunistic pathogen.
  • Global regulators control virulence factor production.
  • The hemB gene's role in S. aureus regulation is not fully understood.

Purpose of the Study:

  • To investigate the regulatory consequences of hemB inactivation in S. aureus Newman strain.
  • To analyze global regulator and virulence factor expression patterns over the growth cycle.

Main Methods:

  • Northern blot analyses were used to examine transcription profiles over 15 hours.
  • hemB and hemB sigB double mutants were created and analyzed.
  • Expression of global regulators (arl, rot, sae, sarR, sarS, srr, svrA, sigB, agr) and virulence factors (hla, spa, sarC, clfA, asp23, rsbVW-sigB) was monitored.

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Main Results:

  • hemB inactivation led to a small-colony phenotype and altered global regulator expression.
  • Transcripts for most global regulators disappeared post-exponential phase in the hemB mutant.
  • Permanent sigmaB activity was observed in the hemB mutant, with variations suggesting additional regulatory factors.

Conclusions:

  • hemB inactivation significantly impacts S. aureus transcriptional patterns, including global regulators and virulence factors.
  • The study highlights a permanent sigmaB activity in hemB mutants, indicating complex regulatory interactions.
  • This research provides a comprehensive overview of transcriptional dynamics during the growth cycle in S. aureus Newman strain.