Related Experiment Videos
Changes in lung permeability after chronic pulmonary artery obstruction.
Elizabeth M Wagner1, Gulnura Karagulova, John Jenkins
1Department of Medicine and Environmental Health Sciences, Johns Hopkins University, Baltimore, MD, USA. wagnerem@jhmi.edu
Journal of Applied Physiology (Bethesda, Md. : 1985)
|October 22, 2005
Summary
Left pulmonary artery ligation (LPAL) in mice induced new blood vessel growth. This remodeled lung vasculature showed increased permeability to water and protein by 21 days post-LPAL.
Area of Science:
- Pulmonary vascular remodeling
- Angiogenesis research
- Cardiovascular physiology
Background:
- Left pulmonary artery ligation (LPAL) in mice triggers rapid angiogenesis, with systemic vessels penetrating the pleura.
- Angiogenic vessels in other organs are known to exhibit increased permeability.
Purpose of the Study:
- To investigate vascular permeability and functional perfusion in the lung following LPAL.
- To determine the timeline of vascular changes and their impact on lung tissue.
Main Methods:
- Assessed vascular permeability using Evans blue dye extravasation, lung wet-to-dry weight ratio, and lavaged protein.
- Measured radiolabel clearance to evaluate functional lung perfusion.
- Utilized labeled microspheres to assess blood flow changes.
Main Results:
- Functional vasculature with normal tracer clearance was established by 6 days post-LPAL.
- Significant increases in Evans blue-albumin and lung wet-to-dry ratio were observed 21 days after LPAL.
- Increased lavaged protein was noted at both 4 hours and 21 days post-LPAL, indicating persistent permeability changes.
Conclusions:
- Remodeled lung vasculature following chronic pulmonary artery obstruction demonstrates increased permeability to water and protein.
- These findings highlight the functional consequences of LPAL-induced angiogenesis and vascular remodeling.