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Updated: Aug 15, 2026

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography (TLC) Coupled with Gas-Liquid Chromatography (GLC)
Published on: March 18, 2011
Fatty acid content and the functional state of pea chloroplasts under altered gravity
Valentyna Baranenko1, Elizabeth Kordyum
1N.G. Kholodny Institute of Botany, NAS of Ukraine, Kyiv, Ukraine. cell@svitonline.com
Abstract:
The results of the study of the fatty acid content and the functional state of chloroplasts isolated from leaves of pea plants grown during 7 and 14 days in the stationary conditions and under clinorotation (2 rpm) are presented. An increase in the unsaturated fatty acid content occurred after 7-day clinorotation while it insignificantly decreased after more prolonged 14-day clinorotation. A study of the functional state of chloroplasts (the rate of electron transport in photosystems II [PSII] and in photosystem I [PSI] and in the whole photosynthetic electron transport chain) showed its decrease under both terms of clinorotation in comparison with control ones. In addition, 14-day clinorotation caused more significant lowering of the electron transport rate, particularly in PSI. Changes in both the fatty acid content and the electron transport rate are discussed in relation to the activation of lipid peroxidation and the increased production of activated oxygen species in chloroplasts under clinorotation.
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