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Aspergillus niger G proteins: subcellular localization.

X Mérit1, J Frot-Coutaz, R Got

  • 1Laboratoire de Biochimie des Membranes, Université Lyon I, Villeurbanne, France.

FEMS Microbiology Letters
|May 1, 1992
PubMed
Summary
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Researchers identified G proteins in Aspergillus niger, differentiating between cytosolic and membrane-bound forms. These proteins were found in the plasma membrane and endoplasmic reticulum, aiding fungal cell function studies.

Area of Science:

  • Molecular Biology
  • Mycology
  • Cell Biology

Background:

  • The postmitochondrial fraction of Aspergillus niger exhibits significant GTPase activity and GTP binding.
  • Understanding the localization of GTP-binding proteins is crucial for elucidating cellular signaling pathways in fungi.

Purpose of the Study:

  • To characterize the subcellular localization of G proteins within Aspergillus niger.
  • To identify and differentiate between cytosolic and membrane-bound G protein populations.

Main Methods:

  • Subcellular fractionation using sucrose gradient centrifugation.
  • Isolation and iodination of outer proteins from Aspergillus niger protoplasts.
  • Immunological detection assays to identify G protein populations.

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Main Results:

  • Separated a cytosolic and four membranous populations based on density.
  • Identified the fungal plasma membrane fraction, characterized by the absence of typical enzymes.
  • Detected cytosolic G proteins and small G proteins in plasma membrane and endoplasmic reticulum membranes using immunological methods.

Conclusions:

  • Successfully localized cytosolic and membrane-bound G proteins in Aspergillus niger.
  • Established the presence of these proteins in both the plasma membrane and endoplasmic reticulum.
  • Provided insights into the distribution of G proteins, contributing to fungal cell biology research.