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i-Tracker: for quantitative proteomics using iTRAQ.

Ian P Shadforth1, Tom P J Dunkley, Kathryn S Lilley

  • 1Department of Analytical Science and Informatics, Cranfield University, Silsoe, Bedfordshire, UK. i.p.shadforth.s01@cranfield.ac.uk

BMC Genomics
|October 26, 2005
PubMed
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This summary is machine-generated.

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i-Tracker software enables linking quantitative iTRAQ data with popular protein identification tools, offering a valuable advancement for proteomics research. This new tool ensures consistent results with existing methods.

Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Biotechnology

Background:

  • iTRAQ technology allows simultaneous relative protein quantitation across multiple samples.
  • Current quantitation is limited to proprietary software (ProQuant), restricting integration with other identification tools.
  • A need exists for software that links iTRAQ quantitation with widely used protein identification algorithms.

Purpose of the Study:

  • To develop i-Tracker software for extracting iTRAQ reporter ion ratios.
  • To enable seamless integration of iTRAQ quantitative data with peptide identifications from tools like Mascot and Sequest.

Main Methods:

  • Developed i-Tracker software to process non-centroided tandem MS peak lists.
  • Designed i-Tracker to extract reporter ion peak ratios compatible with common identification software formats.

Related Experiment Videos

Main Results:

  • i-Tracker successfully extracts quantitative information from iTRAQ experiments.
  • Results generated by i-Tracker are consistent with those from ProQuant, validating its accuracy.
  • Demonstrated the ability to link iTRAQ quantitative data with peptide identifications from Mascot and Sequest.

Conclusions:

  • i-Tracker provides essential functionality for linking iTRAQ quantitation with popular protein identification tools.
  • The software overcomes limitations of existing tools by offering broader compatibility.
  • i-Tracker is a timely and valuable addition to the proteomics research toolkit.