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Published on: April 12, 2010
Determination of bovine serum albumin by a resonance light-scattering technique with the mixed-complex
Ling Li1, Gongwu Song, Guangrong Fang
1Faculty of Chemistry and Material Science, Hubei University, Wu Han 430062, PR China. waitingll@yahoo.com
Journal of Pharmaceutical and Biomedical Analysis
|October 26, 2005
Abstract:
A mixed-complex of La(Phth)(phen)(3+) was synthesized. Resonance light-scattering characteristics of interaction between La(Phth)(phen)(3+) with bovine serum albumin (BSA) were studied. When BSA was added, aggregation of La(Phth)(phen)(3+) on the molecular surface of bovine serum albumin occurred in the pH 5.5-6.3, resulted in an enhanced resonance light-scattering (RLS) peak at 360 nm. The intensity of resonance light-scattering was found to be proportional to the concentration of BSA.
