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Updated: Aug 15, 2026

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
Published on: June 21, 2018
Negative ion electrospray mass spectrometry of neoflavonoids
Alison N Hulme1, Hamish McNab, David A Peggie
1School of Chemistry, The University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, UK. Alison.Hulme@ed.ac.uk
Abstract:
The electrospray ionisation mass spectra of the neoflavanoids brazilin and hematoxylin are reported in both their reduced (1 and 2, respectively) and their oxidised forms (3 and 4, respectively). In the reduced forms, breakdown pathways under collision induced decomposition (CID) conditions produce fragments characteristic of rings A and C; in their oxidised forms, the fragments are characteristic of rings B and D. The structural assignments of the fragments are substantiated by recording the spectra after deuterium exchange at the hydroxyl groups.
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