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Related Experiment Videos

TOPs and their regulation.

T L Hamilton1, M Stoneley, K A Spriggs

  • 1School of Pharmacy, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.

Biochemical Society Transactions
|October 26, 2005
PubMed
Summary
This summary is machine-generated.

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Cellular ribosome production slows during arrest or starvation by inhibiting ribosomal protein (RP) synthesis. This regulation hinges on the 5’-terminal oligopyrimidine tract (5’-TOP) in RP messenger RNAs (mRNAs).

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Ribosome production significantly decreases upon cell-cycle arrest or nutrient deprivation in mammalian cells.
  • This reduction is partly achieved by coordinated inhibition of ribosomal protein (RP) synthesis.
  • Translational control of RP synthesis is mediated by cis-elements in the 5'-untranslated regions (5'-UTRs) of RP mRNAs.

Purpose of the Study:

  • To explore the mechanism of 5'-terminal oligopyrimidine tract (5'-TOP) mRNA regulation.
  • To investigate upstream signaling pathways and trans-acting factors involved in TOP mRNA regulation.
  • To highlight new findings from cDNA microarray and polysome analysis in TOP mRNA regulation.

Main Methods:

  • Analysis of cis-elements within the 5'-UTRs of RP mRNAs.

Related Experiment Videos

  • Investigation of translational regulation of RP synthesis.
  • Application of cDNA microarray technology coupled with polysome analysis.

    • Main Results:

    • The 5'-TOP, a conserved element in RP mRNA 5'-UTRs, plays a crucial role in regulating RP mRNA translation.
    • Upstream signaling pathways and specific trans-acting factors are involved in controlling TOP mRNA translation.
    • Novel insights into TOP mRNA regulation were gained through combined cDNA microarray and polysome analyses.

    Conclusions:

    • The 5'-TOP element is a key regulator of RP mRNA translation, impacting cellular ribosome biogenesis.
    • Understanding the upstream signaling and trans-acting factors provides a deeper insight into cellular stress responses.
    • Advanced techniques like cDNA microarray and polysome analysis are powerful tools for dissecting complex translational control mechanisms.