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The cytoskeleton adaptor protein ankyrin-1 is upregulated by p53 following DNA damage and alters cell migration

A E Hall1, W-T Lu1, J D Godfrey1

  • 1Medical Research Council (MRC), Toxicology Unit, Leicester, UK.

Cell Death & Disease
|April 8, 2016
PubMed

Insights

The tumor suppressor p53 activates microRNA miR-486 and ankyrin-1 (ANK1) after DNA damage. ANK1 protein levels increase significantly, impacting cell migration and survival in cancer patients.

Area of Science:

  • Molecular biology
  • Cellular biology
  • Genomics

Background:

  • Genome integrity is crucial for cellular function, maintained by surveillance and repair mechanisms.
  • The tumor suppressor protein p53 is central to DNA damage response and cell-cycle control.
  • MicroRNAs (miRNAs) and their host genes play significant roles in cellular processes.

Purpose of the Study:

  • To investigate the downstream targets of p53 following DNA damage.
  • To elucidate the role of miR-486 and its host gene ankyrin-1 (ANK1) in the DNA damage response.
  • To explore the clinical relevance of ANK1 in cancer.

Main Methods:

  • Induction of DNA damage in various cell types.
  • Quantitative analysis of miR-486 and ANK1 expression.
  • Assessment of cell-cycle progression (G1/S transition).
  • Microscopy to analyze actin cytoskeleton structure and cell migration.
  • Correlation analysis of ANK1 expression with patient survival data.

Main Results:

  • p53 induces miR-486 and its host gene ankyrin-1 (ANK1) upon DNA damage.
  • ANK1 protein expression increased over 80-fold, and ANK1 is upregulated by diverse DNA damaging agents.
  • miR-486-5p influences G1/S cell-cycle transition post-DNA damage.
  • ANK1 alters actin cytoskeleton structure, supporting limited cell migration during DNA damage.
  • Elevated ANK1 expression is associated with poorer survival rates in cancer patients.

Conclusions:

  • ANK1 is a significant effector downstream of the p53 pathway in response to DNA damage.
  • miR-486 and ANK1 play distinct but coordinated roles in the cellular response to DNA damage.
  • ANK1 represents a potential prognostic biomarker in cancer.

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