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Published on: August 20, 2012
Production of deuterated zeaxanthin by Flavobacterium multivorum and its detection by resonance Raman and mass
Prakash Bhosale1, Pallavi V Teredesai, Jin Lihong
1Department of Ophthalmology and Visual Sciences, Moran Eye Center, University of Utah School of Medicine, 50 North Medical Drive, Salt Lake City, UT 84132, USA.
Abstract:
Flavobacterium multivorum, a zeaxanthin-producing organism, was grown aerobically in a medium prepared with deuterated water. Atmospheric pressure chemical ionization mass spectrometry (APCI-MS) and resonance Raman spectroscopy (RRS) analysis revealed approximately 75% replacement of hydrogen by deuterium atoms as indicated by the molecular mass cluster at around m/z 600. Deuterated zeaxanthin upon excitation with a 488 nm laser exhibited characteristic resonance Raman vibrational modes at 1,161 and 1,504 cm(-1) as compared to 1,007, 1,159 and 1,525 cm(-1 )for undeuterated zeaxanthin. HPLC/APCI-MS and HPLC/RRS were specific and sensitive with limits of detection of 2.5 pg and 50 ng, respectively.
