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Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
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Kinetics study of KPC-3, a plasmid-encoded class A carbapenem-hydrolyzing beta-lactamase

Jimena Alba1, Yoshikazu Ishii, Kenneth Thomson

  • 1Department of Microbiology, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, Tokyo 1438540, Japan.

Antimicrobial Agents and Chemotherapy
|October 28, 2005
PubMed

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Enzyme kinetics studies the rates of biochemical reactions. Scientists monitor the reaction rates for a particular enzymatic reaction at various substrate concentrations. Additional trials with inhibitors or other molecules that affect the reaction rate may also be performed.
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
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Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
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