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Related Experiment Videos

Effective extraction of elastase from Bacillus sp. fermentation broth using aqueous two-phase system.

Ying Xu1, Guo-qing He, Jing-jun Li

  • 1Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China.

Journal of Zhejiang University. Science. B
|October 28, 2005
PubMed
Summary

This study optimized an aqueous two-phase system (ATPS) for extracting elastase from Bacillus. The PEG/phosphate system efficiently partitioned elastase into the PEG-rich phase, achieving high recovery.

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Area of Science:

  • Biotechnology
  • Biochemistry
  • Enzyme Extraction

Background:

  • Elastase is a valuable enzyme with diverse industrial applications.
  • Efficient and scalable extraction methods are crucial for enzyme production.
  • Bacillus species are known producers of extracellular enzymes like elastase.

Purpose of the Study:

  • To evaluate an aqueous two-phase system (ATPS) for the extraction of elastase from Bacillus sp. EL31410.
  • To investigate and optimize parameters influencing elastase partitioning in ATPS.
  • To establish a rapid and convenient method for elastase recovery.

Main Methods:

  • Aqueous two-phase system (ATPS) utilizing polyethylene glycol (PEG) and phosphate salts (KH(2)PO(4)-K(2)HPO(4)).
  • Investigation of elastase and cell partitioning behavior in PEG/salt systems.

Related Experiment Videos

  • Optimization of ATPS parameters (PEG molecular mass, pH, NaCl concentration, phase forming component concentrations) using response surface methodology.

    • Main Results:

    • The PEG/KH(2)PO(4)-K(2)HPO(4) system demonstrated effective separation, partitioning elastase into the PEG-rich phase and retaining cells in the other phase.
    • System parameters like PEG molecular mass, pH, and NaCl significantly influenced elastase partitioning.
    • Optimal conditions were determined as 23.1% (w/w) PEG 2000 and 11.7% (w/w) KH(2)PO(4)-K(2)HPO(4), yielding a predicted recovery of 89.5%.

    Conclusions:

    • ATPS provides a highly efficient method for elastase extraction from Bacillus.
    • The optimized PEG/phosphate system offers a rapid, convenient, and scalable approach for enzyme recovery.
    • This method holds potential for industrial applications requiring purified elastase.