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Updated: Aug 15, 2026

High-resolution Tandem Mass Spectrometry for Studying Chemical Constituents of Gynura bicolor DC
Published on: February 2, 2024
[Biphenyl ferulate from Glehnia littoralis]
1School of Pharmacy, Changchun College of Traditional Chinese Medicine, Changchun 130021.
Objective:
To investigate the prolyl oligopeptidase inhibitory constituents from Glehnia littoralis.
Methods:
To determine the prolyl oligopeptidase inhibitory activity in vitro of ethanol extract of G. littoralis and its fractions with Yoshimoto's method, to separate the constituents of the active fractions by repeated silica gel column chromatogrphy.
Results:
Two ferulate compounds were isolated from EtOAc-soluble fractions of ethanol extract of G. littoralis, which (100 microg/ml) showed mile activity (inhibitory rate: 50%). Compound I, named glehnilate, was a new biphenyl ferulate. Compound II, identified as 2-(4'-hydroxyphenol)-glycol mono trans-ferulate, was isolated from this plant for the first time. Their structures were determined on the basis of their spectral data.
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