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Related Experiment Videos

Microfluidic device for electric field-driven single-cell capture and activation.

Nicholas M Toriello1, Erik S Douglas, Richard A Mathies

  • 1Department of Chemistry and UC Berkeley/UCSF Joint Graduate Group in Bioengineering, University of California, Berkeley, California 94720, USA.

Analytical Chemistry
|November 1, 2005
PubMed
Summary
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Scientists developed a microchip for precisely capturing and activating single cells. This technology enables selective cell adhesion on electrodes for chemical response analysis.

Area of Science:

  • Microfluidics
  • Biotechnology
  • Cell Biology

Background:

  • Single-cell analysis is crucial for understanding cellular heterogeneity and function.
  • Existing methods for single-cell manipulation can be inefficient or lack precision.
  • Developing novel microfluidic devices for controlled cell handling is an active research area.

Purpose of the Study:

  • To develop and characterize a microchip for directed capture and chemical activation of surface-modified single cells.
  • To demonstrate the selective and sequential capture of multiple cell types.
  • To analyze the functional response of captured single cells to chemical stimuli.

Main Methods:

  • Fabrication of a microfluidic device with interdigitated gold electrodes on a glass substrate within PDMS channels.

Related Experiment Videos

  • Surface modification of cells with thiol functional groups via endogenous RGD receptors.
  • Directed cell adhesion to gold electrodes using an applied electric potential.
  • Optimization of single-cell capture efficiency by varying electric field application duration.
  • Monitoring calcium flux in captured CHO cells using a calcium-sensitive dye upon stimulation with carbachol.

    • Main Results:

    • Achieved maximum single-cell capture efficiency of 63% with a 10-minute electric field application.
    • Demonstrated sequential and selective capture of different cell types on specific electrodes.
    • Successfully analyzed the chemical activation of single captured cells, observing a 220% increase in fluorescence intensity in response to carbachol.
    • Validated the ability to direct the adhesion of living single cells and assess their responses.

    Conclusions:

    • The developed microchip enables precise, directed capture of living single cells on microelectrodes.
    • The system allows for the analysis of single-cell responses to chemical stimuli within a microfluidic environment.
    • This technology holds promise for advancing single-cell analysis and drug screening applications.