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Updated: Aug 15, 2026

Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides
Published on: May 23, 2013
Novel transcription factor zfh-5 is negatively regulated by its own antisense RNA in mouse brain
Yuriko Komine1, Kenji Nakamura, Motoya Katsuki
1Division of Brain Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan.
Abstract:
Here, we report features of a novel transcription factor zfh-5, which we isolated from the mouse brain; in addition to the mRNA, the antisense strand of zfh-5 is also expressed in the developing brain, in a manner complementary to the expression of zfh-5 mRNA. Although most neurons express zfh-5 mRNA soon after their final mitosis, several types of neurons, such as the pyramidal and granule cells in the hippocampus, express the zfh-5 antisense RNA prior to the mRNA expression. Using gene-targeting approach, we showed that this antisense RNA has a negative regulatory role on the expression of zfh-5 mRNA. These observations suggest that, in specific types of neurons, the expression of zfh-5 is additionally regulated by a mechanism depending on this antisense RNA.
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