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Effect of different phenolic compounds on alpha-amylase activity: screening by microplate-reader based kinetic assay
1Institut für Pharmazie, Freie Universität, Berlin, Germany.
Die Pharmazie
|November 2, 2005
Abstract:
The inhibitory effect of different polyphenolic plant compounds on alpha-amylase activity was investigated in vitro. A kinetic assay was performed using 96-well-plates. Acarbose was used as positive control (IC50: 23.2 microM). Some of the tested compounds, occurring in plants traditionally used in anti-diabetic tea species, showed an inhibition of the enzyme in physiological concentrations, e.g. luteolin, tannic acid, and isochlorogenic acid.
