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Published on: December 29, 2023
Interactions of anti-sigma factor antagonists of Mycobacterium tuberculosis in the yeast two-hybrid system
B K Parida1, T Douglas, C Nino
1Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA.
Abstract:
Anti-sigma factor antagonists (anti-anti-sigma factors) play critical roles in regulating the expression of alternative sigma factors in response to specific stress signals. The Clusters of Orthologous Groups (COG) database has identified the existence of six genes, Rv0516c, Rv1364c, Rv1365c, Rv1904, Rv2638 and Rv3687c (grouped under the cluster COG1366), encoding potential anti-sigma factor antagonists in Mycobacterium tuberculosis. These molecules are speculated to regulate the expression of sigma factor SigF of M. tuberculosis in response to stress signals. Since signaling occurs via physical interactions of proteins (protein-protein interaction), we investigated whether the anti-sigma factor antagonists of M. tuberculosis interact with anti-sigma factor RsbW (Rv3287c) or the sigma factor SigF (Rv3286c) in the yeast two-hybrid system. The results revealed that most of the anti-sigma factor antagonists interact with either RsbW or SigF or both. In addition, some anti-sigma factor antagonists also displayed limited interactions between themselves. These interactions suggest that they possibly transduce some signals to SigF and between themselves.
Insights
Six potential anti-sigma factor antagonists in Mycobacterium tuberculosis were studied. Most interact with RsbW or SigF, suggesting a role in stress signal transduction to SigF.
Area of Science:
- Microbiology
- Molecular Biology
- Protein Interactions
Background:
- Anti-sigma factor antagonists regulate alternative sigma factors during stress responses.
- Six potential anti-sigma factor antagonists (COG1366) were identified in Mycobacterium tuberculosis.
- These molecules are hypothesized to control the expression of the sigma factor SigF.
Purpose of the Study:
- To investigate the protein-protein interactions of identified anti-sigma factor antagonists.
- To determine if these antagonists interact with RsbW (anti-sigma factor) or SigF (sigma factor).
Main Methods:
- Yeast two-hybrid system was employed to test for protein interactions.
- Interactions between six putative anti-sigma factor antagonists and RsbW/SigF were assessed.
Main Results:
- Most anti-sigma factor antagonists showed interactions with either RsbW, SigF, or both.
- Some antagonists also exhibited self-interactions.
- These findings support the proposed regulatory roles.
Conclusions:
- The identified anti-sigma factor antagonists likely participate in signal transduction pathways.
- Interactions suggest a mechanism for regulating SigF activity in response to stress.
- Further research can elucidate specific signaling cascades involving these proteins.
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