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Related Experiment Videos

Simple sequence repeat-based consensus linkage map of Bombyx mori.

Xue-Xia Miao1, Shi-Jie Xub, Ming-Hui Li

  • 1Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China.

Proceedings of the National Academy of Sciences of the United States of America
|November 3, 2005
PubMed
Summary

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This summary is machine-generated.

A new silkworm genetic map was created using 518 simple sequence repeat (SSR) markers. This high-density map aids silkworm genotyping and comparative genomics in Lepidoptera.

Area of Science:

  • Genomics
  • Molecular Genetics
  • Insect Genetics

Background:

  • Bombyx mori (silkworm) is economically and culturally significant.
  • Previous silkworm genetic maps lacked high marker density.
  • Understanding silkworm genetic architecture is crucial for breeding and research.

Purpose of the Study:

  • To construct a high-density genetic linkage map for Bombyx mori.
  • To establish SSR linkage groups and assign them to silkworm chromosomes.
  • To create a second-generation consensus genetic map for silkworms.

Main Methods:

  • Employed 518 simple sequence repeat (SSR) markers.
  • Genotyped backcross progeny from specific silkworm crosses.
  • Integrated visible mutations and conserved insect genes.

Related Experiment Videos

  • Utilized cleaved amplified polymorphic sequence (CAPS) markers.

    • Main Results:

    • Established 29 SSR linkage groups, refined to 28.
    • Assigned SSR linkage groups to the 28 silkworm chromosomes.
    • Constructed a consensus map with 3431.9 cM total length.
    • Demonstrated high efficiency for silkworm genotyping.

    Conclusions:

    • The new silkworm genetic map provides a valuable resource for research.
    • The map facilitates high-throughput genotyping and marker-assisted selection.
    • Potential for synteny studies across Lepidoptera and other insect orders.