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Sensitive assay for determining plasma tenofovir concentrations by LC/MS/MS
Tom Delahunty1, Lane Bushman, Courtney V Fletcher
1Antiviral Pharmacology Laboratory, Department of Clinical Pharmacy, School of Pharmacy, University of Colorado Health Sciences Center, Denver, 80262, USA. tom.delahunty@uchsc.edu
Summary
A new LC/MS/MS assay accurately measures tenofovir (TNF) in human plasma. This validated method is useful for pharmacokinetic studies and routine monitoring of TNF levels.
Area of Science:
- Analytical Chemistry
- Pharmacology
Background:
- Tenofovir (TNF) is a crucial antiviral medication.
- Accurate monitoring of TNF plasma concentrations is essential for effective treatment and pharmacokinetic studies.
Purpose of the Study:
- To develop and validate a sensitive and reliable liquid chromatography-tandem mass spectrometry (LC/MS/MS) assay for quantifying tenofovir in human plasma.
- To establish the assay's suitability for routine therapeutic drug monitoring and pharmacokinetic investigations.
Main Methods:
- Development and validation of an LC/MS/MS assay using adefovir as an internal standard.
- Protein precipitation with trifluoroacetic acid, followed by reversed-phase chromatography on a Polar-RP column.
- Detection via ESI MS/MS in positive ion mode with specific ion transitions.
Main Results:
- The assay demonstrated linearity from 10 to 750 ng/ml.
- A minimum quantifiable limit of 10 ng/ml was achieved for 250 microl plasma aliquots.
- The method was validated for EDTA-anticoagulated plasma, heparin-treated plasma, and serum matrices.
Conclusions:
- The developed LC/MS/MS assay is a robust and accurate method for tenofovir determination in human plasma.
- This assay is suitable for routine monitoring of tenofovir plasma levels and supports pharmacokinetic studies.