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Updated: Aug 15, 2026
A Microfluidic Model of Biomimetically Breathing Pulmonary Acinar Airways
Published on: May 9, 2016
Effect of low-bias flow oscillation with partial liquid ventilation on fluoroscopic image analysis, gas exchange, and
Budi Wiryawan1, Mark S Dowhy, Bradley P Fuhrman
1Division of Pediatric Critical Care, The Women and Children's Hospital of Buffalo, State University of New York at Buffalo, NY, USA.
Objective:
To evaluate the effect of low-bias flow oscillation (LBFO) with partial liquid ventilation (PLV) on perfluorochemical evaporation, histopathology, and oxidative tissue damage in an animal model of acute lung injury.
Design:
Prospective, randomized animal study.
Setting:
Research laboratory of a health sciences university.
Subjects:
Twelve New Zealand White rabbits.
Interventions:
Juvenile rabbits were anesthetized, paralyzed, and ventilated through a tracheostomy with either high-frequency oscillatory ventilation or LBFO. Lung injury was induced by repeated saline lavage, after which perflubron was instilled through a side port of the endotracheal tube. Lateral fluoroscopic images were performed at baseline and at various postfill intervals of animals in the high-frequency oscillatory ventilation-PLV and LBFO-PLV groups. The images were digitalized for computer analysis of the Lung Lucency Index, a surrogate marker of perflubron evaporation. Histopathologic evaluation was performed using a lung-injury scoring system. Malondialdehyde was measured in lung homogenates to assess oxidative damage.
Measurements And Main Results:
There were no significant differences in gas exchange and ventilator settings between groups throughout the experiment. At 300 mins, the high-frequency oscillatory ventilation-PLV group had a significantly higher Lung Lucency Index compared with the LBFO-PLV group in both dependent and nondependent lung regions (a high Lung Lucency Index correlates with increased perflubron loss). Malondialdehyde measurements were not different between groups. Animals treated with LBFO-PLV had a lower histopathologic lung-injury score compared with high-frequency oscillatory ventilation-PLV.
Conclusion:
LBFO-PLV is a viable mode of ventilation in a model of acute lung injury and is associated with significant preservation of perflubron in comparison with high-frequency oscillatory ventilation-PLV. The lower evaporative losses during LBFO-PLV were associated with improved histology scores.
