Related Experiment Video
Updated: Aug 15, 2026

Synthesis and Purification of Iodoaziridines Involving Quantitative Selection of the Optimal Stationary Phase for Chromatography
Published on: May 16, 2014
Hydride-based silica stationary phases for HPLC: fundamental properties and applications
Joseph J Pesek1, Maria T Matyska
1Department of Chemistry, San Jose State University, CA, USA. pesek@sjsu.edu
Abstract:
Silica hydride is a recent development in chromatographic support materials for HPLC where hydride groups replace 95% of the silanols on the surface. This conversion changes many of the fundamental properties of the material as well as the bonded stationary phases that are the result of further chemical modification of the hydride surface. The general approach for fabricating the silica hydride and subsequent bonded phases is reviewed. Properties of the silica hydride surface are compared to those of the standard material obtained in the preparation of most commercial HPLC stationary phases. Some unique chromatographic properties of hydride-based phases are described as well as some general application areas where these bonded materials may be used in preference to or have advantages not available from typical stationary phases.
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
High-Performance Liquid Chromatography: Instrumentation
High-Performance Liquid Chromatography: Elution Process
