Related Experiment Video
Updated: Aug 15, 2026
Regulating Schwann Cell Growth by Nanosecond Pulsed Electric Field for Peripheral Nerve Regeneration In Vitro
Published on: May 3, 2024
Biological effects of a software-controlled voltage pulse generator (PhyBack PBK-2C) on the release of vascular
P Ferroni1, M Roselli, F Guadagni
1Department of Experimental Medicine and Pathology, University La Sapienza, Viale Regina Elena 324, 00161 Rome, Italy. Patrizia.Ferroni@sanraffaele.it
Background:
Electrical stimulation (ES) may induce vascular permeability and physiological angiogenesis. ES of rat muscles significantly increases the microvessel density and vascular endothelial growth factor (VEGF) protein levels. Thus, a pilot study was designed to analyze the effects of low-voltage electric impulses on VEGF levels in patients with dystrophic ulcers.
Materials And Methods:
Circulating VEGF levels were analyzed in 9 patients undergoing an ES session with low voltage software-controlled impulses applied through topical transducers (1-9 micros width, 1-420-Hz frequency and 30-120 V strength-100 microA max).
Results:
The session was accompanied by a peak of circulating VEGF (3-10 min from start) in all 9 patients, which was preceded by a rise of TNF-alpha (2-min) and was independently associated with soluble E-selectin levels. Nitric oxide generation was significantly improved on the day after treatment. No hemostatic activation or sustained inflammatory reaction were observed.
Conclusion:
ES may represent a safe method for augmenting VEGF-mediated vascular protection, either directly or by induction of NO.
