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Enterococcus devriesei sp. nov., associated with animal sources.
Pavel Švec1, Marc Vancanneyt2, Joanna Koort3
1Czech Collection of Microorganisms, Faculty of Science, Masaryk University Brno, Tvrdého 14, 602 00 Brno, Czech Republic.
International Journal of Systematic and Evolutionary Microbiology
|November 11, 2005
Summary
Two bovine strains previously identified as Enterococcus raffinosus were re-examined. Genetic and phenotypic analyses revealed they represent a new species, Enterococcus devriesei, distinct from related enterococci.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- Biochemical characterization initially assigned bovine strains LMG 13603 and LMG 14595 to Enterococcus raffinosus.
- The taxonomic status of these strains, along with isolates from a lamprey and a poultry plant, required further investigation.
Purpose of the Study:
- To re-evaluate the taxonomic position of specific Enterococcus strains using molecular and phenotypic methods.
- To determine if these strains represent a known species or a novel bacterial taxon.
Main Methods:
- Phylogenetic analysis based on sequencing of the housekeeping gene pheS (phenylalanyl-tRNA synthase alpha-subunit).
- 16S rRNA gene sequencing to confirm phylogenetic placement within the Enterococcus avium species group.
- Phenotypic and genotypic analyses including whole-cell-protein electrophoresis, (GTG)(5)-PCR fingerprinting, ribotyping, and DNA-DNA hybridization.
Main Results:
- Sequence analysis of pheS and 16S rRNA genes indicated that the four strains occupied a distinct phylogenetic position.
- The strains showed high 16S rRNA gene sequence similarity to the Enterococcus avium group but were closest to Enterococcus pseudoavium (99.8-99.9%).
- Integrated phenotypic and genotypic data supported the classification of these strains as a novel species.
Conclusions:
- The four investigated strains, including bovine isolates LMG 13603 and LMG 14595, are distinct from previously described Enterococcus species.
- A new species, Enterococcus devriesei sp. nov., is proposed, with LMG 14595T as the type strain.