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Updated: Aug 14, 2026

Metagenomic Analysis of Silage
Published on: January 13, 2017
Bacillus ruris sp. nov., from dairy farms
Marc Heyndrickx1, Patsy Scheldeman1, Gillian Forsyth2
1Department of Animal Product Quality, Centre for Agricultural Research-Ghent, Brusselsesteenweg 370, B-9090 Melle, Belgium.
Abstract:
Four novel ellipsoidal spore-forming Bacillus isolates with swollen sporangia, isolated from raw milk and feed concentrate, showed a high level of similarity in SDS-PAGE, fatty acid methyl esters and routine phenotypic tests. However, 16S rRNA gene sequence comparisons showed that this taxon was different from other related Bacillus species, and only a low level of DNA relatedness was found with the closest phylogenetic and phenotypic relative, Bacillus galactosidilyticus. This taxon could be differentiated from B. galactosidilyticus on the basis of morphological differences, stronger acid reactions with a wide range of substrates after 48 h incubation, and qualitative and quantitative differences in fatty acid content. On the basis of these data, a novel species, Bacillus ruris sp. nov., is proposed, with LMG 22866T (=DSM 17057T) as the type strain.
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