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Published on: February 24, 2023
[Activating protein-1 members in response to changes of wall-shear stress in osteoblastic cells]
1Dept. of Oral and Maxillofacial Surgery, The Affiliated Hospital, School of Stomatology, Zhejiang University, Hangzhou 310006, China.
Objective:
To observe activating protein-1 (AP- 1) members in response to changes of wall-shear stress in osteoblastic cells in vitro.
Methods:
Isolated and purified osteoblastic cells from the calvaria of newborn SD rats were cultured and subcultured. The third generation cells were subjected to wall-shear stress of 0.8 Pa, 1.2 Pa, 1.4 Pa and 1.6 Pa separately. Gene expression of the seven AP-1 members were studied before (0 h) and 10 min, 15 min, 30 min, 60 min after treated with wall-shear stress.
Results:
The expression of FosB, c-Fos, c-Jun, JunD and JunB mRNA increased transiently after application of 1.2 Pa wall-shear stress in osteoblastic cells compared to 0.8 Pa , 1.4 Pa and 1.6 Pa stress, and peaked at 15 min.
Conclusion:
Mechanical environment changes in osteoblastic cells induced a dramatic induction of most of the AP-1 members.
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