Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

"Clickable" agarose for affinity chromatography.

Sreenivas Punna1, Eiton Kaltgrad, M G Finn

  • 1Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.

Bioconjugate Chemistry
|November 17, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Liquid-Phase CO<b><sub>2</sub></b> Capture by a Nonaqueous Cooperative Absorption Mechanism.

Journal of the American Chemical Society·2026
Same author

Characterization Standard for <i>In-situ</i> Cryo-electron Tomography.

bioRxiv : the preprint server for biology·2026
Same author

Orthogonal Chemistry Enables Precision Nanoparticle Cofunctionalization for Tuning Immune Stimulation and Antigen Presentation.

Biomacromolecules·2026
Same author

Discovery of a paralog-selective p300 protein degrader with potent anti-cancer activity in hematological malignancies.

Nature communications·2026
Same author

Biological evaluation of amidine derivatives: In vitro cytotoxicity and cellular antioxidant capacity.

PloS one·2026
Same author

Tumor agnostic drug delivery with dynamic nanohydrogels.

Nature communications·2026
Same journal

Iron Single Atom Nanozyme-Mediated GPX4 Inhibitor Delivery for Self-Enhanced Ferroptosis.

Bioconjugate chemistry·2026
Same journal

SpyCatcher-Engineered Ferritin Nanocages Enable Dual-Receptor Targeting for Enhanced Glioma Therapy.

Bioconjugate chemistry·2026
Same journal

One-Pot Synthesis of Functionalized Coumarin Fluorophores Enables Rapid Access to Live-Cell Bioorthogonal Labeling and Microenvironmental Sensing Agents.

Bioconjugate chemistry·2026
Same journal

Enzymatic Synthesis of Glyco-DNA Equipped with Oligosaccharides and Charged Monosaccharides.

Bioconjugate chemistry·2026
Same journal

Electrochemical Tryptophan-Selective Bioconjugation in Neutral Buffer via Cooperative <i>N</i>-Oxyl Radicals.

Bioconjugate chemistry·2026
Same journal

Conjugates of Heme and Intrinsically Disordered Peptides toward the Development of Synthetic Artificial Oxygen Carriers.

Bioconjugate chemistry·2026
See all related articles

Click chemistry efficiently links molecules for affinity chromatography. This study demonstrates functionalized agarose beads using azide and alkyne groups for practical biomolecule purification.

Area of Science:

  • Biochemistry
  • Materials Science
  • Chemical Engineering

Background:

  • Affinity chromatography requires ligands attached to supports for biomolecule purification.
  • Click chemistry, specifically Cu(I)-catalyzed azide-alkyne cycloaddition, offers efficient and selective covalent bond formation.
  • Agarose is a biocompatible material commonly used in chromatography.

Purpose of the Study:

  • To develop a practical method for functionalizing agarose beads with azide and alkyne groups.
  • To demonstrate the utility of these functionalized beads as precursors for affinity chromatography supports.

Main Methods:

  • Synthesis of agarose beads functionalized with terminal alkyne groups.
  • Synthesis of agarose beads functionalized with azide groups.

Related Experiment Videos

  • Demonstration of the stability and reactivity of these functionalized beads.

    • Main Results:

    • Easily prepared agarose beads bearing alkyne and azide groups were successfully synthesized.
    • These functionalized beads are stable under various conditions relevant to chromatography.
    • The functionalized beads serve as practical precursors for creating custom affinity chromatography materials.

    Conclusions:

    • Agarose beads functionalized with azide and alkyne groups are readily prepared.
    • These materials are suitable for creating tailored affinity chromatography supports.
    • This approach provides a versatile platform for biomolecule purification using click chemistry.