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[The effect of sodium doecyl sulfate on Streptococcus sanguis biofilm]
1Department of Oral Medicine, School of Stomatology, Ninth People's Hospital, Shanghai Jiaotong University, China. marui6868@yahoo.com
Purpose:
To investigate the effects of sodium doecyl sulfate on Streptococcus sanguis biofilm.
Methods:
Streptococcus sanguis biofilm was formed on saliva-coated glass(SCG) in a flow culture system, then exposed to different dental plaque control agents for 3,10,30 minutes including A: 300 mmol.L(-1) sodium doecyl sulfate (SDS); B: 2% chlorhexidine combined with 300 mmol.L(-1) SDS; C: 0.3%triclosan combined with 300 mmol.L(-1) SDS. Confocal laser scanning microscopy and vital/dead fluorescent staining technique (vital stained green, dead stained red) were combined to observe biofilm thickness, bacterial density and viability, analysis of variance was used for comparison.
Results:
The biofilm thickness and bacteria density reduced after treatment by 300 mmol.L(-1) SDS. However, there was no significant difference after treatment by 2% chlorhexidine combined with 300 mmol.L(-1)SDS; When treated by 0.3% triclosan combined with 300 mmol.L(-1) SDS, most or all of the bacteria detached from the surface and dispersed.
Conclusions:
The combination of SDS (aimed at detachment) and triclosan (aimed at killing bacteria) may be most effective in controlling biofilm.
