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Updated: Aug 6, 2026
Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement
Published on: September 6, 2011
Orientation and confinement of cells on chemically patterned polystyrene surfaces
S A Mitchell1, A H C Poulsson, M R Davidson
1Advanced Materials & Biomaterials Research Centre, School of Engineering, The Robert Gordon University, Aberdeen AB10 1FR, UK. stephen.mitchell@rgu.ac.uk
Abstract:
UV/ozone oxidation was combined with a photomasking technique to produce adjacent regions of different chemistry on polystyrene (PS) surfaces. The surface chemistry and topography were studied using AFM, XPS and contact angle measurements. The physicochemical patterns were visualised by the condensation of water vapour upon the surfaces and by the differential attachment of Chinese hamster ovarian (CHO) cells. The orientation of CHO cells on 55 and 125 microm wide oxidised PS strips were measured and found to be highly dependent on the width of the oxidised feature. CHO cells in relatively close proximity to a linear polar/non-polar border showed significant axial alignment along the border. CHO cells can also be confined to specific regions of the polymer surface. Cells attached to larger areas (75 microm x 75 microm) were found to have a smaller average cell size than cells attached to the smaller (56 microm x 56 microm) areas.
