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Microbial biomass estimation.

R E Madrid1, C J Felice

  • 1Departamento de Bioingeniería, FACET/INSIBIO, Universidad Nacional de Tucumán, Consejo Nacional de Investigaciones Científicas y Técnicas, Tucuman, Argentina. rmadrid@herrera.unt.edu.ar

Critical Reviews in Biotechnology
|November 22, 2005
PubMed
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This review covers biomass quantification methods for bioprocesses. It details techniques for measuring cell mass, including viable cell enumeration and physical methods for accurate bioprocess monitoring.

Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Analytical Chemistry

Background:

  • Automated on-line monitoring and control systems are crucial for optimizing bioprocesses.
  • Biomass is a critical parameter requiring accurate and efficient quantification.
  • Selecting appropriate biomass quantification technology necessitates careful consideration of various factors.

Purpose of the Study:

  • To review and discuss diverse methods for biomass quantification in bioprocesses.
  • To present definitions of biomass and biovolume.
  • To explore considerations for active but not culturable cells and technology selection.

Main Methods:

  • Discussion of chemical methods, including bioluminescence for viable cell enumeration.
  • Presentation of photometric methods: fluorescence and scattered light measurements.

Related Experiment Videos

  • Review of reference methods (dry/wet weight, viable/direct counts) and physical methods (flow cytometry, impedancimetric, dielectric techniques).

    • Main Results:

    • Chemical methods have limited current applications but bioluminescence offers selective viable cell enumeration.
    • Photometric, reference, and physical methods provide a range of options for biomass measurement.
    • The review synthesizes information on various techniques, aiding in technology selection.

    Conclusions:

    • Accurate biomass quantification is essential for effective bioprocess monitoring and control.
    • A variety of quantification methods exist, each with specific advantages and applications.
    • Understanding these methods, including considerations for cell viability and type, is key to successful bioprocess management.