Related Experiment Video
Updated: Jul 4, 2026
07:29
Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
Published on: April 22, 2019
Radiation-related cytological changes in oral malignant cells
Ravi Mehrotra1, Neeta Goel, Mamta Singh
1The Departments of Pathology and Preventive Medicine Moti Lal Nehru Medical College, Allahabad. mravi1@sancharnet.in
Indian Journal of Pathology & Microbiology
|November 22, 2005
Summary
This study investigated radiation responses in oral cancer cells using cytology. Fractionated radiotherapy significantly increased radiation-induced cytological changes in squamous cell carcinoma patients.
Area of Science:
- Oncology
- Cytopathology
- Radiotherapy Research
Background:
- Oral cavity carcinoma is a significant health concern in India, accounting for approximately 40% of malignant tumors.
- Cytological assessment of radiation responses in oral cancer cells remains underexplored.
- Understanding cellular changes post-radiotherapy is crucial for treatment efficacy.
Purpose of the Study:
- To evaluate the cytological effects of fractionated radiotherapy on oral squamous cell carcinoma.
- To quantify specific radiation-induced nuclear abnormalities in oral cancer cells.
- To correlate cytological changes with radiotherapy treatment mechanisms.
Main Methods:
- A study involving 102 patients with oral squamous cell carcinoma undergoing fractionated radiotherapy.
- Serial cytological scrape smears collected from tumors before and after irradiation.
- Quantitative analysis of micronucleation, nuclear budding, binucleation, and multinucleation.
Main Results:
- Significant increases observed in micronucleation (1.6 to 14.1), nuclear budding (0.9 to 5.5), binucleation (7.6 to 28.1), and multinucleation (2.4 to 11.7) post-radiotherapy (p<0.001 for all).
- Other observed radiation changes included abnormal nuclear divisions, fibroblast-like cells, nuclear enlargement, and cytoplasmic granulation.
- These cytological responses demonstrated a significant rise following radiotherapy.
Conclusions:
- Fractionated radiotherapy induces significant and measurable cytological changes in oral squamous cell carcinoma.
- The observed nuclear abnormalities and cellular alterations provide insights into radiotherapy's cell-killing mechanisms.
- Cytology is a valuable tool for assessing treatment response in oral cancer radiotherapy.
More Related Videos
07:38Radiation Treatment of Organotypic Cultures from Submandibular and Parotid Salivary Glands Models Key In Vivo Characteristics
Published on: May 17, 2019
05:42Isolation of Cells with Morphological and Spatial Information from Oral Submucous Fibrosis Samples by Laser Capture Microdissection
Published on: August 11, 2023
Related Concept Videos
02:12What is Cancer?
Cells and tissues must meticulously coordinate their activities for the normal functioning of the human body. Therefore, they exhibit socially responsible behavior - resting, growing, dividing, differentiating, or dying - for the organism’s benefit. Cancer arises when cells divide uncontrollably and invade other tissues or organs.
Although people have known about cancer for centuries, it was only in 1761 that Giovanni Morgagni of Padua performed a detailed autopsy of patients who died from...
Although people have known about cancer for centuries, it was only in 1761 that Giovanni Morgagni of Padua performed a detailed autopsy of patients who died from...
02:59Cancer Prevention
Several factors can increase the risk of cancer in an individual. About 50% of cancer cases can be prevented by adopting a healthy lifestyle, regular exercise, eating healthy, and following a modest cancer prevention diet. Epidemiological studies have consistently shown that populations with vegetable and fruit-rich diets have reduced the incidence of cancer. On the other hand, populations who have a diet rich in animal fat, red meat, junk food, or high calories are predisposed to cancer.
Some...
Some...
01:35Mutations
Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
01:30Skin Cancer
Skin cancer is a type of cancer that occurs when there is an abnormal growth of skin cells, usually triggered by damage to the DNA within the skin cells. It is primarily caused by exposure to ultraviolet (UV) radiation from the sun or artificial sources like tanning beds. Skin cancer is the most common type of cancer worldwide, and its incidence continues to rise.
Basal Cell Carcinoma (BCC): BCC is the most common type of skin cancer, accounting for about 80% of cases. It typically develops in...
Basal Cell Carcinoma (BCC): BCC is the most common type of skin cancer, accounting for about 80% of cases. It typically develops in...
01:26Assessment of the Mouth
A thorough mouth assessment, including inspection and palpation of the lips, gums, tongue, tonsils, uvula, and pharynx, is crucial in detecting potential health issues. Diseases ranging from oral cancer to systemic conditions like diabetes could be identified early through careful oral examination. This article provides a detailed guide on conducting a comprehensive mouth assessment.
Mouth Inspection
The inspection begins with visually examining the mouth for symmetry, color, and size.
Mouth Inspection
The inspection begins with visually examining the mouth for symmetry, color, and size.
01:24Cellular Adaptation IV: Dysplasia and Metaplasia
DysplasiaDysplasia refers to abnormal changes in the size, shape, and organization of mature cells, characterized by pleomorphism, nuclear abnormalities, and increased mitotic activity. It commonly affects epithelial tissues, including the cervix, gastrointestinal tract, respiratory mucosa, and endometrium. Although it may occur alongside hyperplasia, dysplasia is not a true adaptive response but a preneoplastic change with potential to progress to cancer.When confined above the basement...