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Protein determination: a comparison of several methods
1Research Institute of Human Bioclimatology, Experimental Gerontology Unit, and Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Abstract:
A comparison of four methods for the determination of total proteins is presented from the following points of view: - sensitivity; - specificity; - amount of work, chemicals, time and equipment needed for the performance of the determination. The following tests have been examined; Tombs' (absorbancy at 210 nm); Waddell's (difference in absorbancy between 215 and 225 nm); Warburg's (absorbancy at 280 nm); Lowry's (absorbancy at 500 nm after the reaction with phenol reagent). The authors recommend Tombs' method for its outstanding sensitivity, specificity and simplicity as the best of the four.
