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Laboratory method for measuring small amounts of tissue glycogen
Biochemistry and Experimental Biology
|January 1, 1980
Summary
This study details a method for quantifying low glycogen levels in small tissue samples. The procedure involves extraction, precipitation, hydrolysis, and colorimetric determination of glycogen as glucose.
Area of Science:
- Biochemistry
- Analytical Chemistry
Background:
- Glycogen quantification is crucial for understanding metabolic disorders.
- Accurate measurement in limited tissue samples presents a challenge.
Purpose of the Study:
- To develop a reliable method for quantitative glycogen determination.
- To enable analysis in small tissue amounts and low glycogen concentrations.
Main Methods:
- Glycogen extraction via boiling potassium hydroxide.
- Ethanol precipitation and sulfuric acid hydrolysis.
- Colorimetric determination of liberated glucose using orthotoluidine.
Main Results:
- The reported procedure is effective for small tissue samples.
- It is particularly suitable for quantifying low glycogen content.
Conclusions:
- This method provides a robust approach for glycogen analysis.
- It addresses the need for sensitive quantification in limited biological specimens.