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Related Experiment Videos

Cationic liposomes for gene delivery.

Sérgio Simões1, Ana Filipe, Henrique Faneca

  • 1University of Coimbra, Laboratory of Pharmaceutical Technology, Faculty of Pharmacy, Portugal. ssimoes@ci.uc.pt

Expert Opinion on Drug Delivery
|November 22, 2005
PubMed
Summary

Cationic liposomes offer a promising, non-viral method for gene therapy delivery. This review explores factors influencing their effectiveness, from formulation to cellular interactions and clinical potential.

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Corrigendum to "Unveiling the impact of membrane fluidity in shaping lipid-based drug delivery systems development." [Biochim. Biophys. Acta (BBA) - Biomembr. Volume 1868, Issue 1, January 2026, 184461].

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Gene Therapy

Background:

  • Viral vectors are traditional gene delivery methods but pose safety concerns.
  • Cationic liposome-DNA complexes (lipoplexes) are emerging as a safer alternative.
  • Understanding lipoplex behavior is crucial for effective gene therapy.

Purpose of the Study:

  • To review parameters governing cationic liposome-DNA complex (lipoplex) biological activity.
  • To analyze the mechanisms of lipoplex-cell interactions and barriers to gene expression.
  • To discuss novel formulations and clinical applications of lipid-based gene delivery systems.

Main Methods:

  • Literature review of cationic liposome-DNA complex formation and function.
  • Analysis of cellular interaction mechanisms and gene expression barriers.

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  • Exploration of new formulation strategies and clinical trial data.

    • Main Results:

    • Lipoplex formation and formulation significantly impact biological activity.
    • Cellular uptake and endosomal escape are key barriers to efficient gene delivery.
    • Novel lipid-based formulations show potential in overcoming current limitations.

    Conclusions:

    • Cationic liposomes represent a viable non-viral gene delivery strategy.
    • Optimizing lipoplex design and understanding cellular interactions are essential for therapeutic success.
    • Further research and clinical studies are warranted to fully realize their potential in gene therapy.