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A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection
09:12

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

Published on: June 15, 2018

[Quality control: sensitivity of the indirect antiglobulin test by filtration]

L Mannessier1, P Y Le Pennec,

  • 1EFS Nord de France, site de Lille, 21, rue Camille-Guérin, BP 2018, 59012 Lille cedex, France. lucienne.mannessier@efs.sante.fr

Transfusion Clinique Et Biologique : Journal De La Societe Francaise De Transfusion Sanguine
|November 22, 2005
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.